Over several years, scientists at Unilever, Clariant, Vantage-Leuna and expert consultants at ERM have developed a tiered, iterative non-animal NGRA framework for worker safety assessment. The framework involves estimating the amount of chemical a worker is exposed to before comparing these estimates with thresholds for bioactivity determined through a variety of in vitro bioactivity assays to make safety decisions. This framework was applied to a real-world case study chemical to demonstrate how NGRA approaches developed for consumer safety can be adapted to assess worker safety and paves the way for a shift in how worker safety assessments are conducted.